When one door closes, another one opens...

Tina K. Machu, Ph.D.

UNT System College of Pharmacy
University of North Texas Health Science Center
3500 Camp Bowie Boulevard
Fort Worth, TX 76107-2699

PERSONAL INFORMATION
Birth date: October 22, 1956
Birthplace: Wharton, TX
Citizenship: United States

EDUCATION
Ph.D. Pharmacology, May 1990
The University of Texas at Austin, College of Pharmacy
Austin, TX
B.S. Pharmacy, 1981
Magna cum laude
The University of Texas at Austin, College of Pharmacy
Austin, TX
Prepharmacy, 1975-77
Texas A&M University
College Station, TX

AWARDS
Chancellor’s Council Award for Research, Texas Tech University Health Sciences Center, 2002
National Research Service Award #1 F32 AA05321-01
NIAAA
1991-1993
American Foundation for Pharmaceutical Education
Predoctoral Fellowship, 1985-88

MEMBERSHIP IN PROFESSIONAL SOCIETIES

  • American Association of Colleges of Pharmacy
  • American Pharmacists Association
  • Texas Pharmacy Association
  • Tarrant County Pharmacy Association
  • Rho Chi
  • Beta Beta Beta
  • Alpha Lambda Delta

PROFESSIONAL EMPLOYMENT

Associate Dean for Academic Affairs, UNT System College of Pharmacy, 2/12-12/16; 6/17 –
present
Interim Dean, UNT System College of Pharmacy, 1/17- 5/17
Interim Dean, UNT System College of Pharmacy, 9/11-12/11
Assistant Dean, Texas College of Osteopathic Medicine, 3/08-2/12
Professor, Dept. of Pharmaceutical Sciences, 9/12- present
Associate Professor, 8/03- 9/12 (tenured 2005), Department of Pharmacology and Neuroscience
University of North Texas Health Science Center, Fort Worth, TX
Associate Professor (tenured), 9/00-8/03
Assistant Professor, 10/94-8/00
Department of Pharmacology
Texas Tech University Health Sciences Center, Lubbock, TX

Postdoctoral Fellow
Advisor: R. Adron Harris, Ph.D., 1993-1994
Advisor: Michael D. Browning, Ph.D., 1989-1993
Department of Pharmacology
University of Colorado Health Sciences Center, Denver, CO

Pharmacist (weekend), 1985-88
Shoal Creek Hospital, Austin, TX

Pharmacist (weekend), 1984-85
Round Rock Hospital, Round Rock, TX

Pharmacist/Manager, 1981-84
Eckerd Drug, Houston, TX

TEACHING EXPERIENCE

University of North Texas Health Science Center
Self-Care 1, 2013 – present
Self-Care 2, 2014 – present
Pharmacogenomics, Pharmacogenetics, and Personalized Medicine, 2014, Course
Director, 2014 – present
Principles of Medicinal Chemistry and Pharmacology, 2014- present, Co-course director,
2014 – present

Integrated Pharmacotherapy: Renal, Eye, Ear, Nose, Throat, and Skin, 2014 – present
Integrated Pharmacotherapy: Endocrinology and Male/Female Health, 2015 – present
Integrated Pharmacotherapy: Respiratory and Gastrointestinal, 2015 – present
Physical Therapy Pharmacology, 2014- 2015
Nervous System 1, 2005- 2012, Course Director, 2007- 2011
Endocrine System II, 2008 – 2013
Gastrointestinal System I, 2008 – 2013
Gastrointestinal System II, 2008 – 2013
Musculoskeletal and Skin System I, 2008 – 2013
Respiratory System II, 2008 – 2013
Fundamentals of Treatment, 2009 – 2013, Course Director, 2009 – 2013
Fundamentals of Behavioral Sciences, 2009
Cardiopulmonary System I, 2012
Physician Assistant Pharmacology, 2008 – 2013
Functional Neuroscience, 2004 – 2010
Basic and Clinical Pharmacology, 2008 – 2012
Neuropharmacology, 2004 – 2009
CORE Curriculum in Biomedical Sciences, 2004- 2007
Receptors, 2007

Texas Tech University Health Sciences Center
Medical Pharmacology, 1994 – 2002
Integrated Neurosciences, 1995
Molecular and Cellular Pharmacology, 1995, 1998, 2001
Autonomic Pharmacology, 1996, 1999, 2002
Neuropsychopharmacology, 1997, 2000, 2003
Physical Therapy Pharmacology, 1998- 2000
Principles of Pharmacology, 1999 – 2002
Physician Assistant Pharmacology, 2000-2002
Foundations for Medical Practice, 2002-2003

Team UMC Wellness Seminar- Pharmacy Continuing Education
Alcohol Ingestion: Physiological and Pharmacological Consequences, 1998

Texas Tech University Health Sciences Center Health-Net Pharmacy Continuing Education
Multiple Drugs Act at the GABAA Receptor, 1995
Alcohol Abuse and Alcoholism, 1997

University of Colorado Health Sciences Center
Dental Hygienist Lecturer: Chemotherapeutic Agents and Anticonvulsants
Psychiatry Resident Lecturer: GABAA Receptors and Benzodiazepines

The University of Texas at Austin College of Pharmacy
Teaching Assistant
Summer 1989- Clinical Pharmacy Laboratory
September 1988- May 1989- Pharmaceutics, Pharmacokinetics Laboratory
September 1985- August 1988- Pharmacology Laboratory
January 1984- August 1985- Clinical Pharmacy Laboratory

Ph.D. STUDENTS
Texas Tech University Health Sciences Center
Major Advisor for Steven J. Coultrap (Ph.D. conferred 1999)
Laboratory Rotation Advisor for Michelle White, 1997
Laboratory Rotation Advisor for Suzan Parhizgar, 2001
Laboratory Rotation Advisor for Brent Hester, 2002
Laboratory Rotation Advisor for Thomas Shiner, 2002
Laboratory Rotation Advisor for Natalie Porter White, 2002
Laboratory Rotation Advisor for Ayman Hamouda, 2003

University of North Texas Health Science Center
Major Advisor for Naomi Oliveira (Ph.D. conferred 2009)
Laboratory Rotation Advisor for Monica Jenschke, 2004
Laboratory Rotation Advisor for David Lim, 2004
Laboratory Rotation Advisor for Tchedre Kissaou, 2004
Laboratory Rotation Advisor for Naomi Oliveira, 2006

M.S. STUDENTS
Texas Tech University Health Sciences Center
Major Advisor for Xiaofei Wen (M.S. conferred 2001)
University of North Texas Health Science Center
Major Advisor for Rashmi Lote (M.S. conferred 2006)

GRADUATE STUDENT COMMITTEES
Texas Tech University Health Science Center
Julius D. Militante, candidacy and thesis committees, M.S. 1996
Raquelli Ganel, candidacy committee, Ph.D. 1997
Minghua Liu, thesis committee, M.S. 2000
Wang Li, candidacy and thesis committees, Ph.D. 2005
Ayman Hamouda, candidacy and thesis committees, Ph.D. 2007

University of North Texas Health Science Center
Sara Taylor, thesis committee, Ph.D. 2005
Nopporn Thangthaeng, candidacy and thesis committees, Ph.D. 2007
Sebum Lee, candidacy and thesis committees, Ph.D. 2007
Tchedre Kissaou, candidacy and thesis committees, Ph.D. 2008
Monica Jenschke, candidacy and thesis committees, Ph.D. 2009
Lorie Gonzalez, candidacy and thesis committees, Ph.D. 2009
Akiko Dohi, candidacy and thesis committees, Ph.D. 2009
Byjung-Jin Kim, thesis committee, Ph.D., 2010
Niki Mirshams, candidacy and thesis committees, Ph.D. 2011
Ethan Poteet, Ph.D., candidacy and thesis committees, Ph.D. 2012
Andrew Payne, Ph.D., candidacy and thesis committees, Ph.D. 2012
Theresa Carbonero, candidacy and thesis committees, Ph.D. 2012

Lonell Smith, M.S. 2008
Melissa Bearden, M.S. 2008
Sandra Longoria, M.S. 2008
Emilia Bittenbinder, M.S. 2008
David Cummings, M.S. 2009
Amanda Oglesby, M.S. 2009
Nathan Fisch, M.S. 2011

University of North Texas
Michael Kennedy, Ed.D. 2010

UNDERGRADUATE STUDENTS
Texas Tech University Health Sciences Center
Michelle Denae Waugh, ASPET student, summer, 1997
James Tarbox, Howard Hughes Medical Institute student, 1997- 2000
Christopher Shanklin, Howard Hughes Medical Institute student, 1999- 2001
Michael Harris, SABRE student, summer, 1999
Jason Hawkins, SABRE student, summer 2001
University of North Texas Health Science Center
Tyesha Link, SMART/McNair student, 2005
Monica Mitcham, JAMP student, 2005
Rosann Govea, SMART/McNair student, 2006
Theresa Carbonera, 2006
Ferrin Ledezma, STARS student, 2007
Ankita Singh, STARS student, 2008

POST-DOCTORAL FELLOWS
Margaret Hamilton Freeman, Ph.D., 1996-1998
Hongwei (Michael) Sun, Ph.D., 1997-1999
Li Ren, Ph.D., 2001-2002
Volodya Hayrapetyan, Ph.D., 2004-2007

COMMUNITY SERVICE TEACHING EXPERIENCE
Aurora, CO Public Schools- Volunteer in the Adult Literacy Program, 1990-1991
Empowerment Program for Women Ex Offenders, Denver, CO- Volunteer in the Adult
Basic Education and GED Preparation Program, 1991-1994

COMMUNITY SERVICE
Board Member, Opening Doors for Women in Need, 6-16 – present
Trinity Episcopal Church Reaching Out Committee, 1/16 – present

UNIVERSITY SERVICE
University of North Texas Health Science Center
Search Committee for Dean, College of Pharmacy, 2016
UNT System College of Pharmacy Co-Curricular Committee, Chair 2016 – 2017
UNTHSC Faculty Grievance and Appeal Committee, alternate, 2015 – 2016
UNT System College of Pharmacy Promotion and Tenure Committee, 2013 – 2016
UNT System College of Pharmacy Program Assessment Committee, 2013 – 2016
UNT System College of Pharmacy Executive Team, 2012 – present
UNT System College of Pharmacy Admissions Committee, Chair, 2012 – 2014, Member
2014 – 2016
UNT System College of Pharmacy Curriculum Committee, Chair, 2012 – 2013, Member
2013 – 2016
UNTHSC CARE Team, 2011-2016
UNTHSC Institutional Review Board, member 2008 – 2016, Vice Chair 2010-2016
UNTHSC Student Fee Advisory Committee, 2014 – 2015
UNTHSC Instructional Evaluation Committee, 2008 – 2014
UNTHSC Faculty Compensation Committee 2013
Dept. of Pharmacology & Neuroscience Graduate Student Oral Qualifying Exam
Committee, 2008 – 2013
UNTHSC Scholarship Committee, 2011-2012
UNTHSC Interprofessional Education Committee, 2011- 2012
Search Committee for Dean, College of Pharmacy, 2011
TCOM Medical Curriculum Committee, 2008 – 2012
President’s Academic Thinking Council, 2008 – 2011
TCOM Medical Student Performance Committee, 2008 – 2010
TCOM Medical Student Admissions, Interviewer and Committee Member, 2004 – 2012,
Team Leader, 2006-2012
TCOM Admissions Policy Advisory Committee, 2010 – 2012
PA Student Admissions, Interviewer, 2006 – 2010, Committee Member 2008 – 2010
UNTHSC Faculty Retention and Awards Committee, 2009
Search Committee for Cell Biology Chair, 2009

TCOM Task Force on Medical Licensing Examination Preparation, 2008
GSBS Promotion and Tenure Committee and Post-Tenure Review, Ad Hoc 2006, 2007,
regular member 2009
Office of Post-doctoral Education, Advising Council, 2006-2008
Dept. of Pharmacology and Neuroscience Faculty Recruitment Committee, 2007 – 2008
GSBS Intramural Research Program, grant review, 2004-2005

Texas Tech University Health Sciences Center
Medical Student Admissions Committee, 1995- 2002
Medical Admissions Committee Workshop for Undergraduates, TTUHSC- El Paso, TX,
9/11/98
Student Affairs Committee, 1998-2003
Graduate Council, 1996-2000
Committee to Evaluate Integrating TTU and TTUHSC Graduate Programs, 1998
Committee to Evaluate Core Courses for the Graduate School of Biomedical Sciences,
1998
Graduate School of Biomedical Sciences Faculty Retreat, May 8-9, 1998
Graduate School of Biomedical Sciences Faculty Retreat, Sept. 24-26, 1998
Grading and Promotions Committee, 1999-2002
Graduate School of Biomedical Sciences Faculty Retreat, June 16-17, 2000
Graduate Student Recruitment Committee, 2000-2002
Tenure and Promotion Committee, 2001- 2003
Post-tenure Review Committee, 2002-2003
Medical Education Oversight Committee, 2002-2003

OFFICES HELD
Secretary, Graduate Council, Texas Tech University Health Sciences Center, 1997-1998
Chair, Student Affairs Committee, Texas Tech University Health Sciences Center, 2000-
2001, 2002-2003
Treasurer, Texas Research Society on Alcoholism, 1997-2006
Vice President, Texas Research Society on Alcoholism, 2007-2010

GRANT REVIEW
American Heart Association, Western Affiliate, 2001-2004
NIAAA, Ad Hoc Review Panel, INIA, July 30-31, 2002
NIAAA, Ad Hoc Review (telephone), March 26, 2003
NIAAA, Ad Hoc Review, Neurotoxicology and Alcohol Study Section, October 20-21,
2003, February 19-20, 2004
NIAAA, Neurotoxicology and Alcohol Study Section, regular member, October 2004-
June 2008
NIAAA, Ad Hoc Review (telephone), November 10, 2004

PREVIOUS RESEARCH SUPPORT (DIRECT COSTS)
American Heart Association Grant-in-Aid, “Ligand Binding Domains in the 5-
HT3 Receptor”, Principal Investigator, Tina K. Machu, Ph.D., $63,636 per year (direct costs), 7/08-6/10.
NINDS, “Ligand Binding Domains in the 5-HT3 Receptor” (RO1 NS43438),
Principal Investigator, Tina K. Machu, $190,000 per year (direct costs), 12/02-11/08
NIAAA, “Alcohol Modulatory Sites in the 5-HT3A Receptor” (RO1 AA12643),
Principal Investigator, Tina K. Machu, $200,000 per year (direct costs), 6/01-3/06.
Alcoholic Beverage Medical Research Foundation, “Identification of alcohol
site(s) of action in the 5-Hydroxytryptamine3 receptor”, 1/96-12/97, Principal
Investigator, Tina K. Machu, $78,161 (direct costs).
NIAAA, “Ethanol and Phosphorylation of the 5-HT3 receptor” (R29 AA10561),
5/1/95-4/30/00, Principal Investigator, Tina K. Machu, $349,999 (direct costs).
Waggoner Center for Alcohol and Addiction Research and Texas Commission on
Alcohol and Drug Abuse (TCADA), “Alcohol Sites in the Human 5-HT3A and 5-HT3B Receptors”, 10/99-9/01, Principal Investigator, Tina K. Machu, $120,000 (direct costs).

INVITED LECTURES

1993 Department of Biochemistry, University of Tennessee Health Sciences Center, Memphis, Tennessee (Invited seminar speaker)
1993 Department of Pharmacology, Northeastern Ohio Universities College of Medicine, Rootstown, Ohio (Invited seminar speaker)
1994 Department of Pharmacology, Texas Tech University Health Sciences Center, Lubbock, Texas (Invited seminar speaker)
1995 Texas Research Society on Alcoholism, Denton, Texas (Symposium speaker)
1995 Department of Biology, Texas Woman’s University, Denton, Texas (Invited seminar speaker)
1996 Department of Microbiology, Texas Tech University Health Sciences Center, Lubbock, Texas (Invited seminar speaker)
1998 Department of Pharmacology, University of North Texas Health Sciences Center, Fort Worth, TX (Invited seminar speaker)
1998 Department of Pharmacology, University of Florida Health Sciences Center, Gainesville, FL (Invited seminar speaker)
1998 Research Society on Alcoholism, Hilton Head, SC (Symposium Chair and Speaker)
2001 Division of Pharmacology and Toxicology, University of Texas, Austin, TX (Invited seminar speaker)
2001 Department of Pharmaceutical Sciences, Texas Tech University Health Sciences Center, Amarillo, TX (Invited Seminar Speaker)
2003 Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, Fort Worth, TX (Invited Seminar Speaker)
2003 Department of Physiology, Texas Tech University Health Sciences Center, Lubbock, TX (Invited Seminar Speaker)

2005 Research Society on Alcoholism, Santa Barbara, CA (Symposium Co-Chair, Co-Organizer, and Speaker)

2005 Society for Neuroscience, Satellite Symposium on 5-HT3A Receptors, Washington, D.C. (Speaker)

PUBLICATIONS

Refereed

Welder, A.A., T.K. Machu, S.W. Leslie, R.E. Wilcox, J. Bradlaw, and D. Acosta. β-adrenergic receptor characterization of postnatal rat myocardial cell preparations. In Vitro 24:771-777, 1988.

Machu, T.K., J.J. Woodward, and S.W. Leslie. Effects of ethanol on inositol 1,4,5-trisphosphate induced calcium release from brain microsomes. Alcohol 6: 431-436, 1989.

Woodward, J.J., T.K. Machu, and S.W. Leslie. Chronic ethanol treatment alters omega-conotoxin and Bay K 8644 sensitive calcium channels in rat striatal synaptosomes. Alcohol 7: 279-284, 1990.

Machu, T.K., R.W. Olsen, and M.D. Browning. Ethanol has no effect on cAMP-dependent protein kinase-, protein kinase C, or Ca2+ -calmodulin- dependent protein kinase II- stimulated phosphorylation of highly purified substrates in vitro. Alcoholism Clin. Exp. Res. 15: 1040-1044, 1991.

Leidenheimer, N.J., T.K. Machu, S. Endo, R.W. Olsen, R.A. Harris, and M.D. Browning. Cyclic AMP-dependent protein kinase decreases gamma aminobutyric acidA receptor –mediated 36Cl- uptake by brain microsacs. J. Neurochem. 57:722-725, 1991.

Dildy-Mayfield, J.E., T.K. Machu, and S.W. Leslie. Ethanol and Voltage- or Receptor-Mediated Increases in Cytosolic Ca2+ in Brain Cells. Alcohol 9: 63-69, 1991.

Menez, J.-F., T.K. Machu, B.-J. Song, M.D. Browning, and R.A. Deitrich. Phosphorylation of cytochrome P450IIE1 (CYP2E1) by Ca2+ -calmodulin dependent protein kinase, protein kinase C, and cAMP dependent protein kinase. Alcohol and Alcoholism. 28: 445-451, 1993.

Machu, T.K., J.A. Firestone, and M.D. Browning. Ca2+ -calmodulin dependent protein kinase II and protein kinase C phosphorylate a synthetic peptide corresponding to a sequence that is specific for the γ2L subunit of the GABAA receptor. J. Neurochem. 61: 375-377, 1993.

Machu, T.K., R.W. Olsen, and M.D. Browning, Immunochemical characterization of the β2 subunit of the GABAA receptor. J. Neurochem., 61: 2034-2040, 1993.

Machu, T.K. and R.A. Harris. Alcohols and anesthetics enhance the function of 5-
Hydroxytryptamine3 receptors expressed in Xenopus laevis oocytes, J. Pharmacol. Exp. Ther. 271: 898-905, 1994.

Valenzuela, C.F., T.K. Machu, R.M. McKernan, P.Whiting, B.B. VanRenterghem, J.L. McManaman, S.J. Brozowski, G.B. Smith, R.W. Olsen and R.A. Harris. Tyrosine kinase phosphorylation of GABAA recptors. Brain Res. 31: 165-172, 1995.

Harris, R.A., Mihic, S.J., Dildy-Mayfield, J.E., and Machu, T.K. Actions of anesthetics on ligand-gated ion channels: role of receptor subunit composition. FASEB J. 9: 1454-1462, 1995.

Machu, T.K. Alcohols and the anesthetic, halothane, enhance glycine receptor function. Neuroscience-Net 1: article #10004, 1996.

Mascia, M.P., Machu, T.K., and Harris, R.A. Enhancement of homomeric glycine recptor function by long-chain alcohols and anesthetics. Br. J. Pharmacol. 119: 1331-1336, 1996.

Machu, T.K., Mihic, S.J., and Dildy-Mayfield, J.E. Selective actions of a detergent on ligand-gated ion channels expressed in Xenopus oocytes. J. Pharmacol. Exp. Ther. 284: 32-36, 1998.

Machu, T.K. Colchicine competitively antagonizes glycine receptors expressed in Xenopusoocytes. Neuropharmacol., 37: 391-396, 1998.

Chapell, R., Martin, J., Machu, T.K., and Leidenheimer, N.J. Direct channel-gating and modulatory effects of triiodothyronine on recombiant GABAA receptors. Eur. J. Pharmacol. 349: 115-121, 1998.

Machu, T.K., Coultrap, S.J., Waugh, M.D., and Hamilton, M.E. Mutation of putative phosphorylation sites in the 5-Hydroxytryptamine3 receptor does not eliminate its modulation by ethanol. Alcoholism: Clin. Exp. Res. 23: 12-17, 1999.

Coultrap, S.J., Sun, H., Tenner, Jr., T.E., and Machu, T.K. Competitive anatagonism of the mouse 5-HT3 receptor by bisindolylmaleimide I, a selective protein kinase C inhibitor, J. Pharmacol. Exp. Ther. 290: 76-82, 1999.

Sun, H., McCardy, E.A., Machu, T.K., and Blanton, M.P. Characterization of the interaction of 3,4,5-trimethoxybenzoic acid 8-(diethylamino) octyl ester (TMB-8) with the Torpedo californica nicotinic acetylcholine receptor and the 5-HT3 receptor. J. Pharmacol. Exp. Ther. 290: 129-135, 1999.

Sun, H. and Machu, T.K. Bicuculline antagonizes 5-Hydroxytryptamine3A and α2 glycine receptors expressed in Xenopus oocytes. Eur. J. Pharmacol. 391: 243-249, 2000.

Machu, T.K., Hamilton, M.E., Frye, T.F., Shanklin, C.L., Harris, M.C., Sun, H., Tenner, Jr., T.E., Soti, F.S., and Kem, W.R. Benzylidene analogs of anabaseine display partial agonist and antagonist properties at the mouse 5 Hydroxytryptamine3A receptor. J. Pharmacol. Exp. There. 299: 1112-1117, 2001.

Coultrap, S.J. and Machu, T.K. Enhancement of 5-Hydroxytryptamine3A receptor function by phorbol 12-myristate, 13-acetate is mediated by protein kinase C and tyrosine kinase activity. Receptors and Channels 8: 63-70, 2002.

Freeman, T.L., Thiele, G.M., Tuma, D.J., Machu, T.K. and Mailliard, M.E. ATA2-Mediated Amino Acid Uptake Following Partial Hepatectomy is Regulated by Redistribution to the Plasma Membrane. Arch. Biochem. Biophys. 400: 215-222, 2002.

Davies, D.L., Machu, T.K., Guo, Y., and Alkana, R.L. Ethanol sensitivity in ATP-gated P2X receptors is subunit dependent. Alcoholism: Clin. Exp. Res. 26: 773-778, 2002.

Das, P., Bell-Horner, C.L., Machu, T.K. and Dillon, G.H. The GABAA receptor antagonist picrotoxin inhibits 5-hydroxytryptamine type 3A receptors. Neuropharmacol. 44:431-438, 2003.

Sikes, S.S., Hamilton, M.E., Liu, L.-X., Lovinger, D.M., and Machu, T.K. A mutation in the second transmembrane domain of the 5-HT3A receptor stabilizes the open state and alters alcohol modulatory actions. J. Pharmacol. Exp. Ther., 306: 595-604, 2003.

McBride, W.J., Lovinger, D.M., Machu, T., Thielen, R.J., Rodd, Z.A., Murphy, J.M., Roache, J.D., and Johnson, B.A. Serotonin-3 receptors in the actions of alcohol, alcohol reinforcement, and alcoholism. Alcoholism: Clin. Exp. Res. 28:257-267, 2004.

Hayrapetyan, V., Jenschke, M., Dillon, G.H., and Machu, T.K. Co-expression of the 5-HT3B subunit with the 5-HT3A receptor reduces alcohol sensitivity, Mol. Brain Res. 142: 146-150, 2005.

Davies, D.L., Asatryan, L., Kuo, S.F., Woodward, J.J., King, B.F. King, Alkana, R.L., Xiao, C., Ye, J.H., Sun, H., Zhang, L., Hu, X.-Q., Hayrapetyan, V., Lovinger, D.M., and Machu, T.K. The Effects of ethanol on ATP-gated purinergic and 5-HT3 receptors. Alcoholism: Clin. Exp. Res. 30: 349-358, 2006.

Hamouda, A.K., Sanghvi, M., Sauls, D., Machu, T.K., and Blanton, M.P. Assessing the lipid requirements of the Torpedo californica nicotinic acetylcholine receptor. Biochem. 45: 4327-4337, 2006.

Hu, X.-Q., Hayrapetyan, V., Gadhiya, J,. Rhubottom, H.E., Lovinger, D.M., and Machu, T.K. The role of Leucine 293 in transmembrane II of the 5-HT3A receptor in alcohol modulatory actions. Br. J. Pharmacol. 148: 88-101, 2006.

Machu, T.K., Dillon, G.H., Huang, R.-Q., Lovinger, D.M., and Leidenheimer, N.J. Temperature: an important experimental variable in studying PKC modulation of ligand-gated ion channels. Brain Res. 1086: 1-8, 2006.

Zhang, R., White, N.A., Soti., F.S., Kem, W.R., and Machu, T.K. N-terminal domains in mouse and human 5-Hydroxytryptamine3A receptors confer partial agonist and antagonist properties to benzylidene analogs of anabaseine. J. Pharmacol. Exp. Ther. 317: 1276-1284, 2006.

Zhang, R., Wen, X., Militante, J.M., Hester, B., Rhubottom, H.E., Sun, H., Leidenheimer, N.H., Yan, D., White, M.M., and Machu, T.K. The role of Loop F residues in determining differential d-tubocurarine potencies in mouse and human 5-Hydroxytryptamine3A receptors, Biochem. 46: 1194-1204, 2007.

de Oliveira-Pierce, A.N., Zhang, R. and Machu, T.K. Colchicine: a novel positive allosteric modulator of the human 5-Hydroxytryptamine3A receptor, J. Pharmacol. Exp. Ther., 329: 838-847, 2009.

de Oliveira-Pierce, A.N., Lote, R., Zhang, R., White, M.M., and Machu, T.K. Loop C is the major determinant of differential d-tubocurarine affinity in human 5-Hydroxytryptamine3A receptors. In preparation for submission to Biochem.

Machu, T.K. Therapeutics of 5-HT3 receptor antagonists: current uses and future directions. Pharmacol. Ther. 130: 338-347, 2011.

Nonrefereed

Machu, T.K. and Coultrap, S.J. Streamlined site-directed mutagenesis with the Pharmacia U.S.E. kit. Pharmacia Biotech Science Tools 2: 16-17, 1997.

BOOK CHAPTERS

Arias, H.R., T.K. Machu, J.R. Trudell and M.P. Blanton. Current perspectives of general anesthetic action in ligand-gated ion channel receptors. In: Recent Research Developments in Biophysical Chemistry, C.A. Condat and Ana Baruzzi, eds., Research Signpost, India, 2002, pp.123-154.

Mayfield, R.D., Popp, R.L., Machu, T.K., London, E.D., Grant, S.J., Morgan, M.J., and Zukin, S.R. Neurobiology of Substance Abuse. In: Neuropsychiatry, Second Edition, R.B Schiffer, S.M. Rao, and B.S. Fogel, eds., Lippincott, Williams, and Wilkins, Philadelphia, PA, 2003, pp. 840-892.

ABSTRACTS

Welder, A.A., T.K. Machu, S.W. Leslie, R.E. Wilcox, J. Bradlaw and D. Acosta. 125I-iodopindolol binding in postnatal primary myocardial cell cultures, myocyte suspensions, and whole heart membrane preparations. J. Mol. And Cell. Cardiol. 19 (Supplement IV)S41, 1987.

Welder, A.A., T.K. Machu, S.W. Leslie, J. Bradlaw, and D. Acosta. Comparisons of KC1-stimulated and receptor-mediated Ca2+ entry into primary myocardial cell cultures and suspensions. In Vitro Abstracts 23: 56A, 1987.

Machu, T.K., J.J. Woodward, and S.W. Leslie. The effects of ethanol on cerebellar microsomal calcium uptake and inositol 1,4,5-trisphosphate induced calcium release. Abstracts, Texas Pharmacology Meeting, 1989.

Machu, T.K., J.J. Woodward, and S.W. Leslie. Effects of ethanol on inositol 1,4,5-trisphosphate induced calcium release in brain microsomes. Abstracts, Federation of American Societies for Experimental Biology Meeting, 1989.

Machu, T.K., R.W. Olsen, and M.D. Browning. Effects of ethanol on GABAA receptor phosphorylation. Alcoholism Clin. and Exp. Res. 15:352, 1991.

Menez, J.-F., T.K. Machu, B.-J. Song, M.D. Browning, and R.A. Deitrich. Phosphorylation of cytochrome P450IIE1 by Ca2+ -calmodulin dependent protein kinase protein kinase C, and cAMP dependent protein kinase. Alcoholism Clin. And Exp. Res. 15: 352, 1991.

Machu, T.K., R.W. Olsen, and M.D. Browning. GABAA receptor antibodies raised against a β2 subunit-specific synthetic peptide of the receptor. Soc. Neurosci. Abstr., vol. 17, part 1, p. 262, 1991.

Leidenheimer, N.J., T.K. Machu, S. Endo, R.W. Olsen, R.A. Harris, and M.D. Browning. cAMP dependent protein kinase decreases GABAA receptor- mediated 36C1- uptake by brain microsacs. Soc. Neurosci. Abstr., vol. 17, part 1, p. 262, 1991.

DeLorey, T.L., S. Endo, T.K. Machu, M.D. Browning, and R.W. Olsen. Identification of β2 and β3 subunits of the GABAA receptor from rat brain with subunit subtype specific antibodies. Soc. Neurosci. Abstr., vol. 18, part 1, p. 263, 1992.

Machu, T.K., R.W. Olsen, and M.D. Browning. Characterization of subunits of the GABAA receptor phosphorylated by cAMP dependent protein kinase A and protein kinase C. Alcoholism: Clin. And Exp. Ther. 16: 377, 1992.

Machu, T.K., R.K. Freund, M.R. Palmer, and M.D. Browning. Phosphorylation of the β2 subunit of the GABAA receptor in situ in brain slices. Alcoholism: Clin. and Exp. Ther. 17: 477, 1993.

Machu, T.K. and Harris, R.A. Alcohols and anesthetics potentiate glycine receptor responses. Alcoholism: Clin. and Exp. Ther. 18: 517, 1994.

Machu, T.K., S.J. Mihic, X.-P. Qian, and J.E. Dildy-Mayfield. A detergent, Cytoclean has selective actions on ligand-gated ion channels. Soc. Neurosci. Abstr., vol. 21, part 3, p.2059, 1995.

Coultrap, S.J. and Machu, T.K. Site-directed mutagenesis of potential phosphorylation sites in the 5-HT3 receptor. Abstracts, Texas Research Society on Alcoholism, 1996.

Machu, T.K., Strahlendorf, J., and Kem, W.R. Nicotinic receptor ligands antagonize 5-HT3 receptors expressed in Xenopus oocytes. Soc. Neurosci. Abstr., vol. 22, part 3, p. 1780, 1996.

Coultrap, S.J. and Machu, T.K. Phorbol ester modulation of 5-HT3 receptor function. Soc. Neurosci. Abstr., vol. 22, part 3, p. 1780, 1996.

Mascia, M.P., Machu, T.K., and Harris, R.A. Enhancement of homomeric glycine receptor function by long chain alcohols and anesthetics. Soc. Neurosci. Abstr., vol. 22, part 2, p. 1563, 1996.

Coultrap, S.J. and Machu, T.K. Mutation of putative phoshorylation sites in the 5-HT3 receptor does not eliminate its modulation by ethanol. Alcoholism: Clin. Exp. Res. 21: 7A, 1997.

Coultrap, S.J., Lovinger, D.M., and Machu, T.K. Phorbol ester modulation of serotonin 5-HT3 receptor function. Soc. Neurosci. Abstr., vol. 24, part 1, p. 1099, 1998.

Lovinger, D.M., Yu, Y., and Machu, T.K. Involvement of a residue in TM2 of the 5-HT3 receptor in the actions of trichloroethanol. Alcoholism: Clin. Exp. Res. 23: 49A, 1999.

Sun, H., Coultrap, S.J., and Machu, T.K. Chimeras of mouse and human 5-Hydroxytryptamine3 receptors reveal two domains responsible for curare sensitivity. Biophys. J. 76: A334, 1999.

Tarbox, J.A., Sun, H., Field, T.R., and Machu, T.K. Mutation of GLY288Ser in the 5-HT3 receptor enhances ethanol sensitivity. Abstracts, Texas Research Society on Alcoholism, 1999.

Lovinger, D.M., Dixit, M., and Machu, T.K. Cysteine substitution in the pore region of the 5-HT3 receptor- channel complex. Soc. Neurosci. Abstr. vol. 25, part 1, p. 201, 1999.

Sessoms-Sikes, S.S., Yu, Y., Machu, T.K., and Lovinger, D.M. Mutation in the TM2 membrane of the 5-HT3 receptor alters both kinetic properties of the ion channel and sensitivity to trichloroethanol. Soc. Neurosci. Abstr. vol. 25, part 2, p. 1327, 1999.

Tarbox, J.A., Field, T.R., and Machu, T.K. Mutations in the transmembrane two region of the mouse 5-HT3A receptor alter alcohol sensitivity. Abstracts, Texas Research Society on Alcoholism, 2000.

Tarbox, J.A., Field, T.R., and Machu, T.K. Mutations in the transmembrane two region of the mouse 5-HT3A receptor alter alcohol sensitivity. Alcoholism: Clin. Exp. Res. 24: 85A, 2000.

Lovinger, D., Smith, S., Dixit, M., Hustedt, E. and Machu, T. Probing the pore lining region of the 5-HT3 receptor-channel. A three tiered approach to prevention. Alcoholism: Clin. Exp. Res. 24: 85A, 2000.

Das, P., Machu, T.K. and Dillon, G.H. Picrotoxin is a competitive antagonist of the 5-HT3A receptor. Soc. Neurosci. Abstr. vol. 26, part 2, p. 2159, 2000.

Zhang, R., Shanklin, C.L., and Machu, T.K. Comparison of n-chain alcohol sensitivity in mouse and human 5-HT3A receptors. Alcoholism: Clin. Exp. Res. 25: 57A, 2001.

Sessoms-Sikes, J.S., Machu, T.K., and Lovinger, D.M. Mutation in the TM2 membrane of the 5-HT3 receptor alters both kinetic properties of the ion channel and sensitivity to trichloroethanol. Alcoholism: Clin. Exp. Res. 25: 57A, 2001.

Ren, L., Zhang, R. and Machu, T.K. Chimeras of mouse and human 5-HT3A receptors reveal regions involved in differential n-chain alcohol cut-off, Alcoholism: Clin. Exp. Res., 26:107A, 2002.

Davies, D.L., Machu, T.K., Guo, Y., and Alkana, R.L. Ethanol sensitivity in ATP-gated P2X receptors is subunit dependent. Alcoholism: Clin. Exp. Res. 26: 107A, 2002.

Alkana, R.L., Machu, T.K., Guo, Y., Cheung, A., and Davies, D.L. N-chain alcohol “crossover” differs in P2X2 and P2X4 receptors expressed in Xenopus oocytes. Soc. Neurosci. Abstr., CD-ROM, 2002.

White, N.A., Zhang, R., Kem, W.R., and Machu, T.K. Critical domains for ligand binding in the 5-Hydroxytryptamine3A receptor. Biophys. J. 84: 90a, 2003.

Hu, Xiang-Qun, Machu, T.K., and Lovinger, D.M. Role of Leucine 293 in alcohol modulatory actions on 5-HT3A receptors. Alcoholism: Clin. Exp. Res. 28: 60A, 2004.

Zhang, R. and Machu, T.K. Loop C residues in 5-HT3A receptors interact to confer curare potency. Biophys. J. 88: 617a, 2005.

Hayrapetyan, V., Jenschke, M., Dillon, G.H. and Machu, T.K. Comparison of alcohol actions at mouse 5-HT3A and 5-HT3A/B receptors. Alcoholism: Clin. Exp. Res. 29:143A, 2005.

Hu, X.-Q., Machu, T., and Lovinger, D.M. Modulation of 5-HT3A receptors by alcohols: role of leucine 293. Alcoholism: Clin. Exp. Res. 29:143A, 2005.

Hayrapetyan, V. and Machu, T.K. Alcohol actions at mouse 5-HT3A receptors containing mutations at F281 in transmembrane two. Soc. Neurosci Abstr., CD-ROM, 2005.

Hayrapetyan, V. and Machu, T.K. The A subunit of the 5-HT3A/B receptor has inequivalent roles in the two ligand binding domains of the receptor. Soc. Neurosci. Abstr., CD-ROM, 2006.

Zhang, R., Rhubottom, H.E., and Machu, T.K. Actions of trichloroethanol in 5-HT3A receptors containing mutations at selected transmembrane two positions. Alcoholism: Clin. Exp. Res. 31: 204A, 2007.

Sanghvi, M., Hamouda, A.K., Srivastava, S., Davis, M.I., Machu, T.K., Lovinger, D.M., Cohen, J.B., and Blanton, M.P. Identifying the lipid-protein interface of the 5-HT3AR: Hydrophobic photolabeling studies with affinity purified 5-HT3ARs. Biophys. J. 94:1326, 2008.

de Oliveira-Pierce, A. N. and Machu, T.K. The role of loops C and F in interspecies differential pharmacology of the 5-HT3A receptor. FASEB J. 22:lb624, 2008.

Lovinger, D.M., Sanghvi, M., Hamouda, A.K., Srivastava, S., Davis, M.I., Morton, R., Luo, G., Chiara, D.C., Machu, T.K., Cohen, J.B., and Blanton, M.P. Purification and photo-affinity labeling of the 5-HT3A receptor. Alcoholism: Clin. Exp. Res. 32: 158A, 2008.

de Olivera-Pierce and Machu, T.K. The role of Loops C and F residues in ligand recognition in the 5-Hydroxytryptamine3A receptor. Soc. Neurosci. Abstr., CD-ROM, 2008.

Machu, T.K. and Ziller, R. What do pre-matriculation variables tell us about student success? American Association of Colleges of Osteopathic Medicine Abstr., AACOM.org., 2009.

Ziller, R., Machu, T.K., and Arvidson, C. What do pre-admission and academic performance variables tell us about student success? American Association of Colleges of Osteopathic Medicine Abstr., AACOM.org., 2010.

White, A., Lubawy, W., Machu, T., Coyle, R., Coyle, D., Penzak, S., Elrod, S., Slovin, E., and Kominski, C. Recruiting students into an assessment culture: faculty and student views on pharmacy assessment strategies. American Association of Colleges of Pharmacy Abstr., 2016.

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